Determination of Streptomyces sp. SAE4034 Antioxidant and Antibacterial Compound and Its Inhibitory Mechanism on Staphylococcus aureus

The genus Streptomyces is known as producers of bioactive compounds that have antibacterial and antioxidant properties. sp. SAE4034 was to produce can inhibit kill pathogenic bacteria, including Staphylococcus aureus, but the group their inhibitory mechanisms were not yet known. ability in producing antioxidants unknown yet. This research aimed determine classification produced by SAE4034, know compounds, observe against S. aureus using cell leakage method. results this show alkaloid, terpenoid, flavonoid, polyphenol. Compound with Rf values 0,40 0,47 which includes into polyphenol 0,72 alkaloid has growth aureus. a very strong activity scavenge DPPH free radical IC50 value 32,14 ppm an enzyme superoxide dismutase amount 63,10 U/mL mechanism extract through inhibition protein membrane synthesis release nucleic acid material cells highest at 2 MIC doses.